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Catalog Number | orb391954 |
---|---|
Category | Proteins |
Description | Recombinant human IL2 protein is produced by our E. coli expression system and the target gene encoding Pro22-Thr153 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | IL2 |
Entrez | 3558 |
UniProt ID | P60568 |
Protein Sequence | MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0 |
Alternative names | Interleukin-2; IL-2; T-cell growth factor; TCGF; A Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
15.4 kDa | |
E.Coli |
Unconjugated | |
95% | |
23.6 kDa | |
Human IL-2 R alpha, His Tag (orb257613) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408). |
Unconjugated | |
95% | |
48.4 kDa | |
Human IL-2 R alpha Protein, Fc Tag (orb257608) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408). |
Unconjugated | |
90% | |
25.4 kDa | |
Human IL-2 R beta Protein, His Tag (orb257259) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |
Unconjugated | |
95% | |
51.0 kDa | |
Human IL-2 R beta, Fc Tag (orb257617) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |
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