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Catalog Number | orb358966 |
---|---|
Category | Proteins |
Description | Recombinant human IL1B active protein |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | > 97% as determined by SDS-PAGE and HPLC. |
MW | 17.3 kDa |
UniProt ID | P01584 |
Protein Sequence | APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
Protein Length | Full Length of Mature Protein |
Source | E.Coli |
Expression System | 117-269aa |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of > 1.0 × 109 IU/mg. |
Expression Region | 117-269aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 µm filtered PBS, pH 7.4 |
Alternative names | IL-1 beta, Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL1B protein (Active)
Unconjugated | |
95% | |
17.5 kDa | |
Human IL-1 beta, premium grade (orb257615) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1). |
Unconjugated | |
95% | |
19.3 kDa | |
Human IL-1 beta, His Tag (orb762423) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1). |
Unconjugated | |
95% | |
43.8 kDa | |
Human IL-1 beta, Fc Tag (orb752327) is expressed from human 293 cells (HEK293). It contains AA Ala 117 - Ser 269 (Accession # P01584-1). |
Unconjugated | |
95% | |
19.3 kDa | |
Mouse IL-1 beta, His Tag (orb762425) is expressed from E. coli cells. It contains AA Val 118 - Ser 269 (Accession # P10749-1). |
Unconjugated | |
95% | |
19.2 kDa | |
Cynomolgus IL-1 beta, His Tag (orb762424) is expressed from E. coli cells. It contains AA Ala 117 - Ser 268 (Accession # P79182-1). |
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