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Catalog Number | orb391916 |
---|---|
Category | Proteins |
Description | Recombinant human IL12B protein is produced by our mammalian expression system and the target gene encoding Ile23-Ser328 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL12B |
Entrez | 3593 |
UniProt ID | P29460 |
Protein Sequence | IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | NKSF2; Interleukin-12 subunit beta; IL-12B; Cytoto Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
61.3 kDa | |
Human IL-12B, Fc Tag (orb257708) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499). |
Unconjugated | |
95% | |
36.6 kDa | |
Human IL-12B, His Tag (orb257709) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1). |
Unconjugated | |
90% | |
49.2 kDa | |
Human IL-12A, Fc Tag (orb257707) is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459). |
Greater than 90% as determined by SDS-PAGE. | |
61.7 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
39.7 kDa & 27.2 kDa | |
Mammalian cell |
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