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Catalog Number | orb426553 |
---|---|
Category | Proteins |
Description | Recombinant of human IL12 (His) protein |
Form/Appearance | Sterile filtered colorless solution. |
Buffer/Preservatives | IL12 protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol. |
Purity | Greater than 90.0% as determined by SDS-PAGE. |
Protein Sequence | IL12A(p35):RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH. IL12B(p40):IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS |
Application notes | Cytokines And Growth Factors |
Source | Baculovirus |
Biological Activity | The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Alternative names | 23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 1 Read more... |
Note | For research use only |
> 90% as determined by SDS-PAGE | |
43 kDa |
> 95% by SDS-PAGE. | |
Recombinant Human IL-12 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg23-Ser219) of human IL-12 (Accession #NP_002178.2/NP_000873.2) fused with a 6��His Tag at the C-terminus(IL12B), Flag tag at the C-terminus(IL12A). |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human IL23 alpha & IL12 beta Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met23-Ser335(Mouse IL-12 beta)&Ile23-Ser328(Human IL-23 alpha)) of Human IL23 alpha & IL12 beta fused with a His Tag and Avi tag at the C-terminal. |
Greater than 97.0% as determined by analysis by SDS-PAGE. | |
Baculovirus |
98.00% | |
41.9 kDa (predicted); 46 kDa (reducing conditions) |