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Catalog Number | orb391912 |
---|---|
Category | Proteins |
Description | Recombinant human IL11 protein is produced by our P. pastoris by recombinant DNA technology. The protein has a molecular mass of approximately 19000 Daltons and is non-glycosylated. The protein is 177 amino acids in length and differs from the 178 amino acid length of native IL-11 by lacking the amino-terminal praline residue. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 2% Glycine, pH 7.2. |
Purity | Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. |
Target | IL11 |
Entrez | 3589 |
UniProt ID | P20809 |
Protein Sequence | PGPPPGPPRV SPDPRAELDS TVLLTRSLLA DTRQLAAQLR DKFPADGDHN LDSLPTLAMS AGALGALQLP GVLTRLRADL LSYLRHVQWL RRAGGSSLKT LEPELGTLQA RLDRLLRRLQ LLMSRLALPQ PPPDPPAPPL APPSSAWGGI RAAHAILGGL HLTLDWAVRG LLLLKTRL |
Source | P. pastoris |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 2% Glycine, pH 7.2. |
Alternative names | Interleukin-11; IL-11; Adipogenesis inhibitory fac Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
19.1 kDa | |
E.Coli |
Unconjugated | |
90% | |
19.9 kDa | |
Human Flt-3 Ligand, His Tag (orb257477) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1). |
Unconjugated | |
95% | |
39.6 kDa | |
Human IL-11 R alpha, His Tag (orb1149284) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ala 370 (Accession # Q14626-1). |
Unconjugated | |
90% | |
19.9 kDa | |
Human Flt-3 Ligand, His Tag (orb511237) is expressed from Baculovirus-Insect cells. It contains AA Thr 27 - Pro 185 (Accession # P49771-1). |
Unconjugated | |
95% | |
18.0 kDa | |
Human Flt-3 Ligand, premium grade (orb257476) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # AAI26294). |
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