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Catalog Number | orb391943 |
---|---|
Category | Proteins |
Description | Recombinant human IL1 alpha protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 159 amino acids with a molecular mass of 18 kD. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | IL1A |
Entrez | 3552 |
UniProt ID | P01583 |
Protein Sequence | SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Alternative names | IL1F1; Interleukin-1 alpha; IL-1 alpha; Hematopoie Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
18.0 kDa | |
E.Coli |
Unconjugated | |
95% | |
62.4 kDa | |
Human IL-18 R1, Fc Tag (orb257584) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # AAH69575). |
Unconjugated | |
95% | |
19.9 kDa | |
Human IL-1 alpha, His Tag (orb867311) is expressed from human 293 cells (HEK293). It contains AA Ser 113 - Ala 271 (Accession # P01583-1). |
Biotin | |
95% | |
39.3 kDa | |
Biotinylated Human IL-18 R1, His, (orb1184767) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # Q13478-1). |
Biotin | |
95% | |
63.9 kDa | |
Biotinylated Human IL-18 R1, Fc, (orb1152438) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # AAH69575.1). |
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