Human IL 7 Protein

• Catalog Number: orb426538
• Category: Proteins
• Description: Recombinant of human,yeast IL 7 protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer.
• Purity: Greater than 98.0% as determined by SDS-PAGE.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu
• Application notes: Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard
• Source: Saccharomyces cerevisiae
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is 2 x 106 units/mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Stability: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Alternative names: Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
• Note: For research use only
• Expiration Date: 6 months from date of receipt.