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Catalog Number | orb426535 |
---|---|
Category | Tools |
Description | Recombinant of human IL 7 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | DCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH |
Source | Escherichia Coli |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.5ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg. |
Storage | Stability: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a concentrated (1mg/ml) solution in water containing 1x PBS pH-7.4. |
Alternative names | Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7. Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 12 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
17.4 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
17.5 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
18.4 kDa | |
Mammalian cell |
Unconjugated | |
90% | |
52.0 kDa | |
Human IL-7 R alpha, Mouse IgG2a Fc Tag (orb402998) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1 (I66T,V138I)). |
Unconjugated | |
95% | |
51.8 kDa | |
Human IL-7 R alpha, Fc Tag (orb1496263) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 (Accession # P16871-1). |
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