Human IL-5 R alpha / CD125 Protein

• Catalog Number: orb257612
• Category: Proteins
• Description: Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125,which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 85%
• Conjugation: Unconjugated
• MW: 37.7 kDa
• Target: IL-5 R alpha
• Source: Human IL-5 R alpha, His Tag (orb257612) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555).
• Expression System: HEK293
• Biological Activity: Loaded Biotinylated Human IL-5, His, on SA Biosensor, can bind Human IL-5 R alpha, His Tag with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
• Expression Region: Asp 21 - Glu 335
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000555
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL5Ra,CD125
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-5 R alpha protein

Loaded Biotinylated Human IL-5, His, Avitag on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. orb257612) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).