Human IL-5 R alpha / CD125 Protein

• Catalog Number: orb1146968
• Category: Proteins
• Description: Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125,which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.
• Reactivity: Human
• Tag: N-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 63.2 kDa
• Target: IL-5 R alpha
• Source: Human IL-5 R alpha, Fc Tag (orb1146968) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Trp 342 (Accession # Q01344-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-5, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 0.6-10 ng/mL (QC tested).
• Expression Region: Asp 21 - Trp 342
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000555.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL5Ra,CD125
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 63.2 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-5, His Tag (Cat. No. orb612148) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag (Cat. No. orb1146968) with a linear range of 0.6-10 ng/mL (QC tested).