Human IL-4 R alpha / CD124 Protein

• Catalog Number: orb545738
• Category: Proteins
• Description: IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 50.4 kDa
• Target: IL-4 R alpha
• Source: Human IL-4 R alpha, Fc Tag (orb545738) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # P24394-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-4, premium grade at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL (QC tested).
• Expression Region: Met 26 - His 232
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000409.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL4R,CD124,IL4RA
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.4 kDa. The protein migrates as 57-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-4, premium grade (Cat. No. orb257598) at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag (Cat. No. orb545738) with a linear range of 1-20 ng/mL (QC tested).