Human IL-4 R alpha / CD124 Protein

• Catalog Number: orb257626
• Category: Proteins
• Description: IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 24.6 kDa
• Target: IL-4 R alpha
• Source: Human IL-4 R alpha, His Tag (orb257626) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-4 R alpha, His Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, , His Tag with a linear range of 2-16 ng/mL (QC tested).
• Expression Region: Met 26 - His 232
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000409.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL4R,CD124,IL4RA
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.6 kDa. The protein migrates as 36-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-4 R alpha protein

Immobilized Human IL-4 R alpha, His Tag (Cat. No. orb257626) at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag, His Tag with a linear range of 2-16 ng/mL (QC tested).