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Catalog Number | orb429407 |
---|---|
Category | Tools |
Description | Recombinant of human IL4 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS |
Source | Escherichia Coli |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. |
Storage | Stability: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | IL-4 was lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
Alternative names | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymp Read more... |
Note | For research use only |
Application notes | Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard |
Expiration Date | 12 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
15.0 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
11.2 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
8.4 kDa | |
E.Coli |
> 96% as determined by SDS-PAGE and HPLC. | |
8.9 kDa | |
E.Coli |
Unconjugated | |
95% | |
24.6 kDa | |
Human IL-4 R alpha, His Tag (orb257626) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1). |
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