Human IL-27 Ra / TCCR Protein

• Catalog Number: orb257609
• Category: Proteins
• Description: Interleukin-27 receptor subunit alpha ( IL27RA) is also known as Cytokine receptor WSX-1, Cytokine receptor-like 1, Type I T-cell cytokine receptor (TCCR), ZcytoR1, IL27RA belongs to the type I cytokine receptor family and type 2 subfamily. IL27RA contains three fibronectin type-III domains. IL27RA is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. IL27RA is receptor for IL27, but requires IL6ST/gp130 to mediate signal transduction in response to IL27. IL27RA involved in the regulation of Th1-type immune responses. IL27RA also appears to be involved in innate defense mechanisms.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 78.7 kDa
• Target: IL-27 Ra
• Source: Human IL-27 Ra, Fc Tag (orb257609) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003).
• Expression System: HEK293
• Biological Activity: Human IL-27 Ra Protein, Fc Tagimmobilized on CM5 Chip can bind Human IL-27 Protein, Fc Tag with an affinity constant of 3.67 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Gln 33 - Arg 514
• Endotoxins: 1.0 EU per μg
• NCBI: AAH28003
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Alternative names: IL27RA,CRL1,TCCR,WSX1
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 78.7 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-27 Ra protein

Human IL-27 Ra Protein, Fc Tag (Cat. No. orb257609) immobilized on CM5 Chip can bind Human IL-27 Protein, Fc Tag with an affinity constant of 3.67 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).