Human IL-27 Ra / TCCR Protein, His Tag (MALS verified)

• Catalog Number: orb1786179
• Category: Proteins
• Description: Interleukin-27 receptor subunit alpha ( IL27RA) is also known as Cytokine receptor WSX-1, Cytokine receptor-like 1, Type I T-cell cytokine receptor (TCCR), ZcytoR1, IL27RA belongs to the type I cytokine receptor family and type 2 subfamily. IL27RA contains three fibronectin type-III domains. IL27RA is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. IL27RA is receptor for IL27, but requires IL6ST/gp130 to mediate signal transduction in response to IL27. IL27RA involved in the regulation of Th1-type immune responses. IL27RA also appears to be involved in innate defense mechanisms.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 54.2 kDa
• Target: IL-27 Ra
• Source: Human IL-27 Ra Protein, His Tag (orb1786179) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003.1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-27 Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-27 Ra Protein, His Tag with a linear range of 0.008-0.25 μg/mL (QC tested).
• Expression Region: Gln 33 - Arg 514
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 54.2 kDa. The protein migrates as 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-27 Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-27 Ra Protein, His Tag (Cat. No. orb1786179) with a linear range of 0.008-0.25 μg/mL (QC tested).