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Catalog Number | orb257627 |
---|---|
Category | Proteins |
Description | Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 26.9 kDa |
Target | IL-21 R |
Source | Human IL-21 R, His Tag (orb257627) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9HBE5-1). |
Expression System | HEK293 |
Biological Activity | Immobilized Biotinylated Human IL-21, Fc, at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-21 R, His Tag with a linear range of 0.8-3 ng/mL (QC tested). |
Expression Region | Cys 20 - Pro 236 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_068570.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL21R,CD360,NILR Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.9 kDa. The protein migrates as 37-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-21 R protein
Immobilized Biotinylated Human IL-21, Fc, Avitag at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (0.5 μg/well) plate can bind Human IL-21 R, His Tag (Cat. No. orb257627) with a linear range of 0.8-3 ng/mL (QC tested).
Unconjugated | |
95% | |
51.5 kDa | |
Human IL-21 R, Fc Tag (orb257628) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9HBE5-1). |
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