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| Catalog Number | orb1087555 |
|---|---|
| Category | Proteins |
| Description | Human IL-2 Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1). |
| Target | IL-2 |
| Conjugation | Unconjugated |
| Tag | N-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | P60568 |
| MW | 41.9 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.9 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Ala 21 - Thr 153 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

Immobilized Human IL-2 R alpha, His Tag (Cat. No. orb257613) at 5 μg/mL (100 μL/well) can bind Human IL-2 Protein, Fc Tag (Cat. No. orb1087555) with a linear range of 0.1-2 ng/mL (QC tested).
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 51.4&75.7 KDa. Observed: 54-66&98-140 KDa, reducing conditions |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.6 kDa after removal of the signal peptide. | |
Mammalian |
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