Human IL-1RL1 / ST2 Protein

• Catalog Number: orb257583
• Category: Proteins
• Description: Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 61.0 kDa
• Target: IL-1RL1
• Source: Human IL-1RL1, Fc Tag (orb257583) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # NP_003847).
• Expression System: HEK293
• Biological Activity: Loaded Human IL-1RL1, Fc Tag on Protein A Biosensor, can bind with Biotinylated Human IL-33, His,an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Lys 19 - Pro 323
• Endotoxins: 1.0 EU per μg
• NCBI: NP_003847
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-1RL1 protein

Loaded Human IL-1RL1, Fc Tag (Cat. No. orb257583) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His, Avitag an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).