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Catalog Number | orb426440 |
---|---|
Category | Proteins |
Description | Recombinant of human IL 13 Variant protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.2, containing 5% trehalose. |
Purity | Greater than 95% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GQFN |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be 1,000,000 units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 13 Variant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized Interleukin-13 Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 Variant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Interleukin-13, NC30, ALRH, BHR1, P600, IL-13, MGC Read more... |
Note | For research use only |
> 95% by SDS-PAGE and HPLC analyses. | |
Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13. | |
Escherichia coli. |
Greater than 95.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 95% as determined by RP-HPLC and analysis by SDS-PAGE |