Human IL-12 R beta 1 / CD212 Protein

• Catalog Number: orb570282
• Category: Proteins
• Description: The human IL-12 R subunit is a member of the cytokine receptor superfamily and the functional high-affinity IL-12R is composed of at least two beta-type cytokine receptor subunits, each independently exhibiting a low affinity for IL-12. IL-12 R beta 1 (Interleukin-12 receptor subunit beta-1) is also known as IL-12RB1, CD212. Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 83.5 kDa
• Target: IL-12 R beta 1
• Source: Human IL-12 R beta 1, Fc Tag (orb570282) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1).
• Expression System: HEK293
• Biological Activity: Human IL-12 R beta 1, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Cys 24 - Glu 540
• Endotoxins: 1.0 EU per μg
• NCBI: NP_005526.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL-12 R beta 1,IL12RB1,CD212,IL12R,IL12RB,IL-12RB1
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 83.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).