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Catalog Number | orb867311 |
---|---|
Category | Proteins |
Description | Human IL-1 alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 113 - Ala 271 (Accession # P01583-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 19.9 kDa |
Target | IL-1 alpha |
UniProt ID | P01583 |
Protein Sequence | NP_000566.3 |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-1 RI Protein, Fc Tag (Cat. No. ILI-H5253) at 10 μg/mL (100 μL/well) can bind Human IL-1 alpha, His Tag (Cat. No. ) with a linear range of 0.3-2.5 μg/mL (QC tested). |
Expression Region | Ser 113 - Ala 271 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000566.3 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IL-1 RI Protein, Fc Tag at 10 μg/mL (100 μL/well) can bind Human IL-1 alpha, His Tag (Cat. No. orb867311) with a linear range of 0.3-2.5 μg/mL (QC tested).
98.00% | |
93.4 kDa (predicted) |
Greater than 90% as determined by SDS-PAGE. | |
E.coli |
> 95% by SDS-PAGE. | |
Recombinant Human IL-1 alpha/IL1A/IL1F1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser113-Ala271) of human IL-1 alpha/IL1A/IL1F1 (Accession #NP_000566.3) fused with a 6��His Tag at the C-terminus. |