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Catalog Number | orb347313 |
---|---|
Category | Antibodies |
Description | Human IgM (Fc5u) antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Human IgM Fc5µ fragment |
Concentration | 1.5 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. |
Conjugation | Biotin |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti-Human IgM (Fc5u) Antibody Biotin Conju Read more... |
Note | For research use only |
Application notes | Anti-Human IgM Fc5µ Biotin antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:190,000 is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Biotin | |
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation, ion exchange chromatography followed by tandem molecular sieve chromatography and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti- Human IgM, and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Papain. | |
Human |
ELISA, FC, IHC, WB | |
Human | |
Goat | |
Polyclonal | |
Biotin |
ELISA, FC, IHC, WB | |
Goat | |
Polyclonal | |
Biotin |
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