Human IgG4 Fc Protein

Catalog Number: orb257573

Catalog Number	orb257573
Category	Proteins
Description	Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
Reactivity	Human
Tag	Native
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	25.8 kDa
Target	IgG4 Fc
Protein Sequence	Glu 99 - Lys 327
Source	Human IgG4 Fc, Tag Free (orb257573) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861).
Expression System	HEK293
Expression Region	Glu 99 - Lys 327
Endotoxins	1.0 EU per μg
Storage	-20℃
Buffer/Preservatives	Tris with Glycine, Arginine and NaCl, pH7.5
Alternative names	IGHG4 Read more...
Note	For research use only
Application notes	This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
Expiration Date	6 months from date of receipt.

SDS-PAGE analysis of Human IgG Fc protein

Human IGHG4 protein [orb706966]
Applications:
ELISA, MS, SDS-PAGE, WB
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Source:
Human cells
Sizes Available: 500 μg, 50 μg, 10 μg
Human IGHG4 protein [orb706967]
Applications:
ELISA, MS, SDS-PAGE, WB
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Source:
Human cells
Sizes Available: 500 μg, 50 μg, 10 μg
Human IgG4 Fc protein [orb669707]
Source:
Human Cells
Sizes Available: 1 mg, 500 μg, 50 μg, 10 μg

Purity:	> 95% by SDS-PAGE.
Source:	KMP1732, Recombinant Human IgG4 Fc Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Glu99-Gly326) of human IgG4 Fc (Accession #P01861).

Sizes Available: 500 μg, 50 μg

Human IgG4 Fc protein [orb420027]
Source:
Human Cells
Sizes Available: 1 mg, 500 μg, 50 μg, 10 μg

Applications:	ELISA, MS, SDS-PAGE, WB
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Source:	Human cells