Human IgG2 Fc Protein

• Catalog Number: orb257567
• Category: Proteins
• Description: Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 25.7 kDa
• Target: IgG2 Fc
• Protein Sequence: Glu 99 - Lys 326
• Source: Human IgG2 Fc, Tag Free (orb257567) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 326 (Accession # P01859-1).
• Expression System: HEK293
• Expression Region: Glu 99 - Lys 326
• Endotoxins: 1.0 EU per μg
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGHG2
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 25.7 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IgG Fc protein