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Catalog Number | orb257567 |
---|---|
Category | Proteins |
Description | Human IgG2 Fc, Tag Free is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 326 (Accession # P01859-1). |
Target | IgG2 Fc |
Conjugation | Unconjugated |
Tag | Native |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | Glu 99 - Lys 326 |
UniProt ID | P01859 |
MW | 25.7 kDa |
Application notes | This protein carries no "tag". The protein has a calculated MW of 25.7 kDa. The protein migrates as 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Glu 99 - Lys 326 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
SDS-PAGE analysis of Human IgG Fc protein
> 90% as determined by SDS-PAGE | |
25.7 kDa |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |