Human IgG (gamma chain) Antibody Rhodamine Conjugated

SKU: orb347270

Description

Human IgG (gamma chain) antibody (TRITC)

Images & Validation

• Tested Applications: FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG (gamma chain) Rhodamine has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG gamma heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, and Human Serum. Minimal cross reactivity was observed by ELISA against other Human heavy and light chain proteins.
• Conjugation: TRITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-Human IgG (gamma chain) rhodamine conjugated Antibody, rabbit anti-Human IgG Antibody TRITC conjugation

Western Blot of Rhodamine conjugated Rabbit anti-Human IgG (gamma chain) secondary antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Human IgG (gamma chain). Other band(s): none.