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Catalog Number | orb347211 |
---|---|
Category | Antibodies |
Description | Human IgG (gamma chain) antibody |
Clonality | Polyclonal |
Species/Host | Goat |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid (sterile filtered) |
Concentration | 1.0 mg/mL |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Human IgM or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes. |
Immunogen | Human IgG gamma heavy chain |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Application notes | Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
Antibody Type | Secondary Antibody |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Alternative names | Goat Anti Human IgG (gamma chain) Antibody, Goat A Read more... |
Note | For research use only |
702 Peptides are printed in duplicates randomly distributed on the microarray. Control peptides (HA and FLAG controls) are located in a square surrounding the peptides of interest. As secondary antibody DyLight™ 549 conjugated goat anti-human IgG antibody and for the FLAG control peptide a mouse anti-FLAG-Cy3 antibody were used.