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Catalog Number | orb346653 |
---|---|
Category | Antibodies |
Description | Human IgG F(c) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Human IgG F(c) fragment |
Concentration | 10.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | None |
Alternative names | rabbit anti-Human IgG F(c) fragment Antibody, rabb Read more... |
Note | For research use only |
Application notes | Anti-Human IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
DOT | |
Texas Red | |
Human IgG F(c) Texas Red was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
DOT, ELISA, SDS-PAGE, WB | |
Unconjugated | |
HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
DOT | |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
DOT, ELISA, IHC, WB | |
Biotin | |
Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
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