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| Catalog Number | orb426385 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human IGF1 protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | The protein was lyophilized from a concentrated solution in PBS, pH 7.0. |
| Purity | Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
| Application notes | Cytokines And Growth Factors |
| Source | Escherichia Coli |
| Biological Activity | The biological activity was determined by the cell proliferation assay using serum free human MCF-7 cells in 5.0 x 105IU/mg. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized IGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA MGF. |
| Note | For research use only |
Greater than 90% as determined by SDS-PAGE. | |
34.7 kDa | |
E.coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
7.3 KDa | |
Mammalian |
IF, IHC, IP, WB | |
Bovine, Gallus, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Bovine, Gallus, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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