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Catalog Number | orb426385 |
---|---|
Category | Proteins |
Description | Recombinant of human IGF1 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The protein was lyophilized from a concentrated solution in PBS, pH 7.0. |
Purity | Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The biological activity was determined by the cell proliferation assay using serum free human MCF-7 cells in 5.0 x 105IU/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized IGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA MGF. |
Note | For research use only |
Greater than 95% as determined by reducing SDS-PAGE. | |
7.3 KDa | |
Mammalian |
ELISA, FA, HPLC, SDS-PAGE, WB | |
Unconjugated | |
> 95% pure by SDS-PAGE and HPLC analyses. | |
9.1 kDa |
Greater than 90% as determined by SDS-PAGE. | |
34.7 kDa | |
E.coli |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 110-120 (Alpha subunit) and 52-55 (Beta subunit) kDa based on Tris-Bis PAGE result. |