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Catalog Number | orb391899 |
---|---|
Category | Proteins |
Description | Recombinant human IGF1 protein is produced by our E. coli expression system and the target gene encoding Gly49-Ala118 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 300mM NaAc, pH 6.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | IGF1 |
Entrez | 3479 |
UniProt ID | P05019 |
Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 300mM NaAc, pH 6.5. |
Alternative names | IBP1; Insulin-like growth factor I; IGF-I; Mechano Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
129.3 kDa | |
Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
Greater than 95% as determined by reducing SDS-PAGE. | |
7.3 KDa | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
34.7 kDa | |
E.coli |
ELISA, FA, HPLC, SDS-PAGE, WB | |
Unconjugated | |
> 95% pure by SDS-PAGE and HPLC analyses. | |
9.1 kDa |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 110-120 (Alpha subunit) and 52-55 (Beta subunit) kDa based on Tris-Bis PAGE result. |