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Catalog Number | orb426389 |
---|---|
Category | Proteins |
Description | Recombinant of human IGF1 N15 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | IGF1 N15 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IGF1 N15 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized IGF1 N15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 N15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Alternative names | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano Read more... |
Note | For research use only |