Human IGF-I Protein, His Tag

SKU: orb1146986

Description

Human IGF-I, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019-1).

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 9.5 kDa. The protein migrates as 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: IGF-I
• Reactivity: Human
• Tag: N-10×His
• Molecular Weight: 9.5 kDa
• Expression Region: Gly 49 - Ala 118
• Purity: 90%
• Endotoxins: 0.1 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Disclaimer: For research use only

Biotinylated Human IGF-I R, His, Avitag immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade (Cat. No. orb1146986) with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Quick Database Links

Gene Symbol

IGF-I

UniProt

P05019