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Human IgA protein

Catalog Number: orb346223

DispatchUsually dispatched within 5-10 working days
$ 350.00
Catalog Numberorb346223
CategoryProteins
DescriptionHuman IgA protein
IsotypeIgA
ConjugationUnconjugated
Form/AppearanceLiquid (sterile filtered)
Concentration1.0 mg/ml
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityHuman IgA whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Human IgA whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
Tested applicationsSDS-PAGE
Dilution rangeELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Application notesHuman IgA whole molecule has been tested in SDS-Page and Western blot and can be utilized as a control or standard reagent in SDS, Western Blotting and ELISA experiments.
SourceHuman
Biological OriginHuman
StorageStore vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Alternative namesHuman Immunoglobulin A
NoteFor research use only
Human IgA protein

Identification of b12A2 antibody secreted in the milk of transgenic mice using western blotLanes 1–8 are milk samples collected from individual mice; lane 9 is the molecular weight marker; lane 10 is milk from a negative control mouse; lane 11 is a known concentration of standard human IgA (p/n orb2652753) at 80 ng/lane; lane 12 is negative control mouse milk spiked with standard human IgA. 80 ng of IgA standard is equivalent to 0.8 mg/ml in milk when loaded at 0.1 µl of milk per lane. Reactive bands were detected using biotinylated goat anti-human IgA alpha chain (p/n orb347244) followed by HRP conjugated streptavidin-IR800CW.

Human IgA protein

SDS-PAGE of Human IgA Whole Molecule. Lane 1: Human IgA Whole Molecule, Reduced [5.0 µg]. Lane 2: Human IgA Whole Molecule, Reduced [1.0 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Human IgA Whole Molecule, Non-Reduced [1.0 µg]. Lane 5: Human IgA Whole Molecule, Non-Reduced [5.0 µg]. 4-20% Gel, Coomassie Blue Stained. Expected MW: ~160 kDa Non-Reduced, ~55kDa, and 23kDa Reduced. Additional bands: monomer, dimer and trimer.

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    0.5 mg
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    FACS,  IF,  IHC-P

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    20 μg, 100 μg
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    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
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    IF,  IHC-Fr,  IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    200 μg, 50 μl, 100 μl, 200 μl
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    ELISA

    Virus

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    50 μl, 500 μl