Human IgA protein (FITC)

SKU: orb346233

Description

Human IgA protein conjugated to FITC

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT
• Application Notes:
  Human IgA whole molecule Fluorescein has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Source: Human
• Biological Origin: Human
• Biological Activity: 4.2
• Isotype: IgA
• Purity: This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgA and anti-Human Serum. No reaction was observed against anti-Human IgM Fc5µ or anti-Human IgG F(c).
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

Human IgA fluorescein conjugated, Human IgA FITC conjugation

Dot Blot of Human IgA Fluorescein Antigen: Human IgA Fluorescein Load: 3-fold serial dilution starting at 200 ng Block: orb348637 for 30 min at RT