Human IgA protein (Biotin)

SKU: orb346256

Description

Human IgA protein conjugated to Biotin

Images & Validation

• Application Notes:
  Human IgA whole molecule Biotin is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Source: Human
• Biological Origin: Human
• Isotype: IgA
• Purity: This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgA (a chain specific) and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Human IgM (Fc5µ).
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

Human IgA Whole Molecule Biotin Conjugate

SARS-CoV-2 N protein amino acid coverage on SARS-CoV-2 microarray. (A) Final N protein constructs fabricated on the SARS-CoV-2 microarray, including the full-length protein, core domain (amino acids 44-362), N-terminal domain (NTD) (amino acids 43-179), C-terminal domain (CTD) (amino acids 246-363), peptide 6 (amino acids 395-412), peptide 8 (amino acids 211-228) and peptide 16 (amino acids 367-389). (B) Final microarray layout: 24-plex array with 16 probes (summarized in the Table insert) printed in triplicate. Control antigens used in the microarray included 50 µg/ml of biotinylated human immunoglobulins G, A, and M (hIgG, hIgA, and hIgM, respectively) and 132 µg/ml of biotinylated anti-human immunoglobulin G (anti-hIgG) as well as in house derivatized NHS-ester-Cy3 biotinylated BSA (Cy3-BSA) at 40 µg/ml.