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Human IFN-gamma Protein
Catalog Number: orb257562
| Catalog Number | orb257562 |
|---|---|
| Category | Proteins |
| Description | Human IFN-gamma, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # P01579). |
| Target | IFN-gamma |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | AAH70256 |
| UniProt ID | P01579 |
| MW | 16.8 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 16.8 kDa. The protein migrates as 15 kDa, 19 kDa and 24 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.01 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Human IFN-gamma, premium grade at 5 µg/mL (100 µL/well) can bind Human IFN-gamma R1, Fc Tag with a linear range of 1-10 ng/mL (QC tested). |
| Expression Region | Gln 24 - Gln 166 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Cytokines |
| Note | For research use only |
| NCBI | AAH70256 |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IFN gamma protein
Immobilized Human IFN-gamma, premium grade (Cat. No. orb257562) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. orb257559) with a linear range of 1-10 ng/mL (QC tested).
- Item 1 of 1
Human IFN gamma protein (Active) [orb359214]
> 98% as determined by SDS-PAGE and HPLC.
16.9 kDa
E.Coli
500 μg, 100 μg - Item 1 of 1
Human IFN gamma protein [orb594774]
Greater than 95% as determined by SDS-PAGE.
16.88 kDa
E.coli
10 μg, 50 μg, 500 μg, 1 mg - Item 1 of 2
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- Item 1 of 2
Human IFNGR1 Protein, hFc Tag [orb1291003]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide. The apparent molecular mass of IFNGR1-hFc is approximately 55-100 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg
