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Human IFN-alpha / beta R1 Protein, Fc Tag
Catalog Number: orb257558
| Catalog Number | orb257558 |
|---|---|
| Category | Proteins |
| Description | Human IFNAR1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620). |
| Target | IFN-alpha/beta R1 |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 90% |
| Protein Sequence | NP_000620 |
| UniProt ID | P17181 |
| MW | 73.8 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.8 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. ) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Lys 28 - Lys 436 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000620 |
SDS-PAGE analysis of Human IFN-alpha/beta R1 protein
Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
