Human IFN-alpha 1 Protein

• Catalog Number: orb1147003
• Category: Proteins
• Description: Interferon alpha-1 is also known as IFN-alpha-1/13, Interferon alpha-D, LeIF D and IFNA1, belongs to the alpha / beta interferon family. Interferons alpha-1 and alpha-13 have identical protein sequences. Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha can either suppress or promote the development of autoimmune diabetes. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 45.8 kDa
• Target: IFN-alpha 1
• Source: Human IFN-alpha 1, Fc Tag (orb1147003) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
• Expression System: HEK293
• Biological Activity: Loaded Human IFN-alpha / beta R1, His Tag on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
• Expression Region: Cys 24 - Glu 189
• Endotoxins: 1.0 EU per μg
• NCBI: NP_076918.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
• Alternative names: IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Loaded Human IFN-alpha / beta R1, His Tag (Cat. No. orb867302) on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. orb1147003) with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).