Human IFN-alpha 1 Protein, His Tag

• Catalog Number: orb735041
• Category: Proteins
• Description: Human IFN-alpha 1, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
• Target: IFN-alpha 1
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• UniProt ID: P01562
• MW: 21.3 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Cys 24 - Glu 189
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).