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Catalog Number | orb80386 |
---|---|
Category | Proteins |
Description | Recombinant of Human IFN a 2a protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 99.0% as determined by both:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg |
Source | Nicotiana Sp.Plant |
Biological Activity | The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 × 108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells. |
Storage | Stability: Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized, containing 50% SDS. |
Alternative names | Leukocyte interferon, B cell interferon, Type I in Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by reducing SDS-PAGE. | |
19.24 KDa | |
Mammalian |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 98.0% as determined by both(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Greater than 97.0% as determined by both:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |