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Catalog Number | orb391874 |
---|---|
Category | Proteins |
Description | Recombinant human ICAM1 protein is produced by our mammalian expression system and the target gene encoding Asn26-Glu480 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | ICAM1 |
Entrez | 3383 |
UniProt ID | P05362 |
Protein Sequence | NAQTSVSPSK VILPRGGSVL VTCSTSCDQP KLLGIETPLP KKELLLPGNN RKVYELSNVQ EDSQPMCYSN CPDGQSTAKT FLTVYWTPER VELAPLPSWQ PVGKNLTLRC QVEGGAPRAN LTVVLLRGEK ELKREPAVGE PAEVTTTVLV RRDHHGANFS CRTELDLRPQ GLELFENTSA PYQLQTFVLP ATPPQLVSPR VLEVDTQGTV VCSLDGLFPV SEAQVHLALG DQRLNPTVTY GNDSFSAKAS VSVTAEDEGT QRLTCAVILG NQSQETLQTV TIYSFPAPNV ILTKPEVSEG TEVTVKCEAH PRAKVTLNGV PAQPLGPRAQ LLLKATPEDN GRSFSCSATL EVAGQLIHKN QTRELRVLYG PRLDERDCPG NWTWPENSQQ TPMCQAWGNP LPELKCLKDG TFPLPIGESV TVTRDLEGTY LCRARSTQGE VTRKVTVNVL SPRYEVDDIE GRMDEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Intercellular adhesion molecule 1; ICAM-1; Major g Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 75.7 kDa after removal of the signal peptide.The apparent molecular mass of ICAM-1-mFc is approximately 90-120 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |