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Catalog Number | orb391872 |
---|---|
Category | Proteins |
Description | Recombinant human HYAL1 protein is produced by our Mammalian expression system and the target gene encoding Phe22-Trp435 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | HYAL1 |
Entrez | 3373 |
UniProt ID | Q12794 |
Protein Sequence | FRGPLLPNRP FTTVWNANTQ WCLERHGVDV DVSVFDVVAN PGQTFRGPDM TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD FSGLAVIDWE AWRPRWAFNW DTKDIYRQRS RALVQAQHPD WPAPQVEAVA QDQFQGAARA WMAGTLQLGR ALRPRGLWGF YGFPDCYNYD FLSPNYTGQC PSGIRAQNDQ LGWLWGQSRA LYPSIYMPAV LEGTGKSQMY VQHRVAEAFR VAVAAGDPNL PVLPYVQIFY DTTNHFLPLD ELEHSLGESA AQGAAGVVLW VSWENTRTKE SCQAIKEYMD TTLGPFILNV TSGALLCSQA LCSGHGRCVR RTSHPKALLL LNPASFSIQL TPGGGPLSLR GALSLEDQAQ MAVEFKCRCY PGWQAPWCER KSMWVDHHHH HH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5. |
Alternative names | LUCA1; Hyaluronidase-1; Hyal-1; Hyaluronoglucosami Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% | |
Calculated MW of 52.6 kDa | |
Human PH20, Tag Free (orb1146978) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1). |
Unconjugated | |
95% | |
53.0 kDa | |
Human PH20, His Tag (orb257775) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1). |
Greater than 90% as determined by SDS-PAGE. | |
48.1 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
50.1 kDa | |
E.coli |
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