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Catalog Number | orb391852 |
---|---|
Category | Proteins |
Description | Recombinant human HMGB3 protein is produced by our mammalian expression system and the target gene encoding Met1-Glu200 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | HMGB3 |
Entrez | 3149 |
UniProt ID | O15347 |
Protein Sequence | MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | HMG2A; HMG4; High mobility group protein B3; High Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
26.8 kDa | |
Human HMGB1, His Tag (orb257546) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P09429-1). |
Unconjugated | |
95% | |
51.6 kDa | |
Human HMGB1 Protein, Mouse IgG2a Fc Tag (orb545767) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Glu 215 (Accession # P09429-1). |
≥90% as determined by SDS-PAGE | |
This protein contains the human HMGB3(Met1-Glu200) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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