Human HMGB1 protein

SKU: orb391848

Description

Recombinant human HMGB1 protein is produced by our Mammalian expression system and the target gene encoding Gly2-Glu215 is expressed with a 6His tag at the C-terminus.

Research Area

Autophagic, Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: HMGB1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Protein Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDEVDHHH HHH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, 10% Glycerol, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Quick Database Links

Gene Symbol

HMGB1

UniProt

P09429

NCBI Gene ID

3146