Human HMGB1 Protein

• Catalog Number: orb257546
• Category: Proteins
• Description: High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 26.8 kDa
• Target: HMGB1
• Protein Sequence: NP_002119.1
• Source: Human HMGB1, His Tag (orb257546) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P09429-1).
• Expression System: HEK293
• Expression Region: Met 1 - Glu 215
• Endotoxins: 1.0 EU per μg
• NCBI: NP_002119.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: HMGB1,HMG1,HMG3,SBP-1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.8 kDa. The protein migrates as 32 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human HMGB1 protein