You have no items in your shopping cart.
Cart summary
Item 1 of 1
Human HMGB1 Protein, His Tag
Catalog Number: orb257546
| Catalog Number | orb257546 |
|---|---|
| Category | Proteins |
| Description | Human HMGB1, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P09429-1). |
| Reactivity | Human |
| Tag | C-10×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 26.8 kDa |
| Target | HMGB1 |
| UniProt ID | P09429 |
| Protein Sequence | NP_002119.1 |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Met 1 - Glu 215 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_002119.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Buffer/Preservatives | PBS, pH 7.4 |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.8 kDa. The protein migrates as 32 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human HMGB1 protein
- Item 1 of 1
Human HMGB1 Protein, His Tag [orb2321419]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 25.7 kDa after removal of the signal peptide. The apparent molecular mass of HMGB1-His is approximately 15-35 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg 
HMGB1 Protein, Human, Recombinant (aa 57-158, His) [orb1956279]
98.00%
13.6 kDa (predicted); 15 kDa (reducing conditions)
50 μgHMGB1 Protein, Human, Recombinant (aa 1-215, His) [orb1957804]
SDS-PAGE: 98.6%; SEC-HPLC: 97.3%
26.3 kDa (predicted); 30-34 kDa (reducing condition, due to glycosylation)
500 μg, 200 μg, 100 μgRecombinant Human HMGB1 Protein, His Tag [orb1550480]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
Recombinant Human HMGB1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Glu215) of Human HMGB1 fused with a His Tag at the C-terminal.
100 μgHuman HMGB1 Protein, His Tag [orb2701650]
> 93%, determined by SDS-PAGE
This protein contains the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus and expressed from HEK293 Cells.
100 μg, 200 μg
