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Catalog Number | orb391847 |
---|---|
Category | Proteins |
Description | Recombinant human HMBS protein is produced by our mammalian expression system and the target gene encoding Ser2-His361 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | HMBS |
Entrez | 3145 |
UniProt ID | P08397 |
Protein Sequence | SGNGNAAATA EENSPKMRVI RVGTRKSQLA RIQTDSVVAT LKASYPGLQF EIIAMSTTGD KILDTALSKI GEKSLFTKEL EHALEKNEVD LVVHSLKDLP TVLPPGFTIG AICKRENPHD AVVFHPKFVG KTLETLPEKS VVGTSSLRRA AQLQRKFPHL EFRSIRGNLN TRLRKLDEQQ EFSAIILATA GLQRMGWHNR VGQILHPEEC MYAVGQGALG VEVRAKDQDI LDLVGVLHDP ETLLRCIAER AFLRHLEGGC SVPVAVHTAM KDGQLYLTGG VWSLDGSDSI QETMQATIHV PAQHEDGPED DPQLVGITAR NIPRGPQLAA QNLGISLANL LLSKGAKNIL DVARQLNDAH VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | PBGD; UPS; Porphobilinogen deaminase; PBG-D; Hydro Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
39.6 kDa | |
E.Coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human HMBS(Ser2-His361) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
98% | |
47 KDa, reducing conditions |
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