Human HGF Protein

• Catalog Number: orb700640
• Category: Proteins
• Description: Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. In addition, HGF has been implicated in a variety of cancers, including of the lungs, pancreas, thyroid, colon, and breast.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 81.6 kDa (53.7 kDa|27.9 kDa)
• Target: HGF
• Source: Human HGF, His Tag (orb700640) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).
• Expression System: HEK293
• Biological Activity: Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Gln 32 - Ser 728
• Endotoxins: 0.1 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: HGF,HPTA,SF
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. orb700640) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).