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Human HB-EGF Protein
Description
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia Coli |
|---|---|
| Biological Activity | The ED50 was determined by the ability to induce proliferation of 3T3 cells and is 0.13-0.2ng/ml. This corresponds to an expected specific activity of 7.7 x 106units/mg. |
| Protein Sequence | MDLQEADLDL LRVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK YKDFCIHGEC KYVKELRAPS CICHPGYHGE RCHGLSL |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Stability: Lyophilized Human HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
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| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium phosphate pH-7.5. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human proHB-EGF (C-6His) Protein [orb1471751]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
15.1 KDa
Mammalian
50 μg, 10 μgRecombinant Human HBEGF Protein, N-His [orb2967445]
>90% as determined by SDS-PAGE.
17.95 kDa
1 mg, 100 μg, 50 μgHuman HBEGF protein (Active) [orb359173]
> 97% as determined by SDS-PAGE and HPLC.
9.7 kDa
E.Coli
10 μg, 500 μg, 100 μgHuman HBEGF protein [orb755859]
ELISA, WB
Greater than 95% as determined by SDS-PAGE
9.4 kDa
E.Coli
1 mg, 200 μg, 100 μg, 50 μg

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Protocol Information
Human HB-EGF Protein (orb425994)
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