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Human GSS protein

SKU: orb391824

Description

Recombinant human GSS protein is produced by our E. coli expression system and the target gene encoding Ala2-Val474 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceATNWGSLLQD KQQLEELARQ AVDRALAEGV LLRTSQEPTS SEVVSYAPFT LFPSLVPSAL LEQAYAVQMD FNLLVDAVSQ NAAFLEQTLS STIKQDDFTA RLFDIHKQVL KEGIAQTVFL GLNRSDYMFQ RSADGSPALK QIEINTISAS FGGLASRTPA VHRHVLSVLS KTKEAGKILS NNPSKGLALG IAKAWELYGS PNALVLLIAQ EKERNIFDQR AIENELLARN IHVIRRTFED ISEKGSLDQD RRLFVDGQEI AVVYFRDGYM PRQYSLQNWE ARLLLERSHA AKCPDIATQL AGTKKVQQEL SRPGMLEMLL PGQPEAVARL RATFAGLYSL DVGEEGDQAI AEALAAPSRF VLKPQREGGG NNLYGEEMVQ ALKQLKDSEE RASYILMEKI EPEPFENCLL RPGSPARVVQ CISELGIFGV YVRQEKTLVM NKHVGHLLRT KAIEHADGGV AAGVAVLDNP YPVLEHHHHH H
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
DisclaimerFor research use only

Alternative Names

Glutathione synthetase; GSH synthetase; GSH-S; Glutathione synthase

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human GSS protein (orb391824)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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