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Catalog Number | orb49421 |
---|---|
Category | Proteins |
Description | Recombinant human GMCSF protein |
Tag | tag free |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 10mM TrisHCl, 4% Mannitol, 1% Sucrose, pH 8.5. |
UniProt ID | P04141 |
Protein Sequence | Ala18-Glu144 |
Source | P.Pichia |
Expression System | Pichia pastoris |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 10mM TrisHCl, 4% Mannitol, 1% Sucrose, pH 8.5. |
Alternative names | GranulocyteMacrophage ColonyStimulating Factor pro Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
14.6 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
14.4 kDa | |
Yeast |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSF-hFc is approximately 35-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
P. pastoris |
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